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KMID : 0385520130260010019
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Analytical Science & Technology
2013 Volume.26 No. 1 p.19 ~ p.26
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Analysis of gibberellic acid from fruits using HPLC/UV-vis
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Ma Kyung-Na
Cho Hyun-Woo
Myung Seung-Woon
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Abstract
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Gibberllic acid (GA3) is one of gibberellins (GAs) that are a class of plant growth hormones that exert profound and diverse effects on plant growth and development. GA3 is essentially non-UV absorbing and is difficult to assay by UV-detector. For effective extraction of gibberellic acid from fruits by using liquidliquid extraction, optimized pH and extraction solvent were established. The selective and sensitive derivative of GA3 for HPLC/UV-vis was derivatized using phenacyl bromide, and the experimental factors, including reaction time, reaction temperature and amount of derivatizing reagent and base were investigated for the effective synthesis. The derivatized GA3 with phenacyl bromide was effectively analyzed by HPLC/UV-vis. The structure of derivatized GA3 was confirmed by HPLC/ESI-MS. For apple, LOD and LOQ were 0.008 mg/
kg and 0.027 mg/kg, respectively. For pear, LOD and LOQ were 0.003 mg/kg, 0.012 mg/kg, respectively. The established method can be applied to more effective analysis of GA3 from plant and food.
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KEYWORD
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gibberellic acid, plant hormone, HPLC/UV-vis, derivatization
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